Ma bicyclo awiri a isomeri [4.1.0]heptane analogue a glycosidase inhibitor galacto-validamine, (1R*,2S,3S,4S,5S,6S*) -5-amino-1-(hydroxymethyl)bicyclo[4.1.0]heptane -2,3,4-triol, apangidwa mu masitepe 13 kuchokera ku 2,3,4,6-tetra-O-benzyl-D-galactose.Zolepheretsa za ma amine awiri oletsedwa mogwirizana, ndi ma acetamide ofanana nawo, adayesedwa ndi ma enzymes a alpha-galactosidase ochokera ku nyemba za khofi ndi E. coli.Ntchito ya glycosyl hydrolase banja la GH27 enzyme (nyemba ya khofi) idaletsedwa mopikisana ndi 1R,6S-amine (7), kulumikizana komangiriza komwe kumadziwika ndi mtengo wa K (i) wa 0.541 microM.GH36 E. coli alpha-galactosidase inawonetsa kusagwirizana kocheperako kwambiri ndi 1R,6S-amine (IC(50)= 80 microM).Diastereomeric 1S, 6R-amine (9) yomangidwa mofooka ku magalactosidase onse, (nyemba ya khofi, IC(50)= 286 microM) ndi (E. coli, IC(50)= 2.46 mM).
