Zogulitsa

Aptasensor yogwira mtima idapangidwa momwe graphene oxide (GO) idagwiritsidwa ntchito ngati chizindikiritso cha mawonekedwe a electrochemical impedance spectroscopy ndi electrochemiluminescence (ECL) kupanga chizindikiro.Aptasensor adapangidwa podziphatikiza yekha kafukufuku wa ECL wa thiolated adenosine triphosphate binding aptamer (ABA) yolembedwa ndi Ru complex (Ru (bpy)3 (2+) zotumphukira) pamwamba pa golide wa nanoparticle (AuNP) wosinthidwa wagalasi wa carbon. electrode (GCE).ABA yosasunthika pa AuNP yosinthidwa GCE imatha kutsatsa kwambiri GO chifukwa champhamvu ya π-π pakati pa ABA ndi graphene oxide;ECL kuzimitsidwa kwa Ru complex ndiye ikuchitika chifukwa cha kutengerapo mphamvu ndi ma elekitironi kusamutsa, ndi kuwonjezeka kwakukulu kwa electron kusamutsa kukana (Ret) ya elekitirodi.Ngakhale pamaso pa chandamale adenosine triphosphate (ATP), ndi ABA amakonda kupanga ABA-ATP bioaffinity complexes, amene ali ofooka kuyandikana kwa graphene okusayidi ndi kusunga graphene okusayidi kutali elekitirodi pamwamba, motero kulola ndi ECL chizindikiro kuwongola, ndi molumikizana ndi kuchepa kwa Ret.Chifukwa cha mphamvu ya ECL yozimitsa bwino, mawonekedwe apadera, ndi zamagetsi zamagetsi za graphene oxide, mphamvu ya Ret ndi ECL motsutsana ndi logarithm ya ATP ndende inali yozungulira kuyambira 10 pM mpaka 10 nM ndi malire otsika kwambiri a 6.7 pM mpaka 4.8pm, motero.Aptasensor yomwe ikufunsidwayo inawonetsa kuberekana bwino kwambiri, kukhazikika, ndi kusankha kwapadera, ndipo ATP ikhoza kusiyanitsa bwino ndi ma analogi ake.Chofunikira kwambiri, njira yabwinoyi ya ECL aptasensor yozikidwa pa GO ikugwira ntchito ngati electrochemical ndi chizindikiro cha chizindikiro cha ECL ndi yanthawi zonse ndipo imatha kufalikira mosavuta ku zochitika zina zomangirira zamoyo.