Zogulitsa

1.Optically active D-2-hydroxybutanoate ndi chomangira chofunikira chapakati cha mankhwala ndi biodegradable poly(2-hydroxybutanoate).Kinetic resolution ya racemic 2-hydroxybutanoate ikhoza kukhala njira yobiriwira komanso yofunikira pakupanga D-2-hydroxybutanoate.Mu ntchitoyi, D-2-hydroxybutanoate pamtunda waukulu (0.197 M) ndi kuchuluka kwa enantiomeric (99.1%) kunapangidwa ndi NAD-lodziimira L-lactate dehydrogenase (L-iLDH) yomwe ili ndi biocatalyst.2-Oxobutanoate, wapakatikati wina wofunikira, adapangidwanso pamlingo wapamwamba (0.193 M).Pogwiritsa ntchito njira yosavuta yosinthira ion ndi macroporous anion exchange resin D301, D-2-hydroxybutanoate idasiyanitsidwa ndi biotransformation system ndi kuchira kwakukulu kwa 84.7%.

2.Asymmetric synthesis ya amino acid osakhala achilengedwe adawonetsedwa ndi omega-transaminase kuchokera ku Vibrio fluvialis JS17.L-2-Aminobutyric acid idapangidwa kuchokera ku 2-oxobutyric acid ndi benzylamine yokhala ndi enantiomeric ochulukirapo kuposa 99%.Zomwe zidachitikazi zidawonetsa kuletsa kwambiri kwa benzaldehyde, yomwe idagonjetsedwa ndikugwiritsa ntchito njira ya biphasic reaction kuti ichotse choletsacho pagawo lamadzi.Mu mawonekedwe a biphasic reaction (50 mm 2-oxobutyric acid, 70 mM benzylamine ndi 2.64 U/ml yoyeretsedwa enzyme) pogwiritsa ntchito hexane ngati chotsitsa, kutembenuka kwa 2-oxobutyric acid kunafika 96% mu 5 h pomwe 39% yokha kutembenuka kunapezedwa popanda. m'zigawo mankhwala.