Zogulitsa

Matenda a chiwindi amafuta osaledzeretsa (NAFLD) ali ndi matenda ambiri kuyambira pa steatosis yocheperako yomwe imadziwika ndi kusungidwa kwa lipids mkati mwa maselo a chiwindi kupita ku steatohepatitis (NASH) kuwonetsa kuchuluka kwamafuta, kutupa, kuphulika ndi kuwonongeka kwa hepatocytes, ndi fibrosis.Pamapeto pake, steatohepatitis ingayambitse matenda a chiwindi ndi hepatocellular carcinoma. -1,4-dihydrocollidine (DDC) yokhala ndi zakudya.Kusanthula kwa jini ya RNA-Seq, kusanthula kwa mapuloteni ndi mbiri ya kagayidwe kachakudya kunagwiritsidwa ntchito kuti azindikire ma jini/mapuloteni owonetsedwa mosiyanasiyana komanso milingo ya metabolite yosokoneza yachiwindi cha mbewa pamankhwala a DDC.Kuwunika kwa njira kunawonetsa kusintha kwa arachidonic acid (AA) ndi S-adenosylmethionine (SAMe) metabolism panjira zina.Kuti mumvetsetse kusintha kwa kagayidwe kachakudya ka arachidonic acid metabolism potengera mawonekedwe a matenda, njira ya kinetic yanjirayi idapangidwa ndikukonzedwa molingana ndi milingo ya metabolite.Pambuyo pake, chitsanzocho chinagwiritsidwa ntchito pophunzira za silico zotsatira za mankhwala omwe angakhalepo a steatohepatitis.Tinazindikira AA / eicosanoid metabolism yomwe imasokonezeka kwambiri mu mbewa za DDC pogwiritsa ntchito njira yoyesera ndi silico.Kusanthula kwathu kwa njira ya metabolic ya AA/eicosanoid kumasonyeza kuti 5-hydroxyeicosatetraenoic acid (5-HETE), 15-hydroxyeicosatetraenoic acid (15-HETE) ndi prostaglandin D2 (PGD2) amasokonezeka mu mbewa za DDC.Tikuwonetsanso kuti mtundu wosinthika ukhoza kugwiritsidwa ntchito kulosera zakusintha kwa metabolic kutengera deta ya transcriptomics pankhani yokhudzana ndi matenda.Kuphatikiza apo, SAMe metabolism idadziwika kuti ikusokonekera chifukwa cha chithandizo cha DDC.Majini angapo komanso ma metabolites a gawoli akuwonetsa kusiyana pakati pa A/J ndi C57BL/6J mbali imodzi ndi PWD/PhJ mbali inayo.