X-GAL CAS: 7240-90-6 98% Yoyera mpaka yoyera Crystalline Powder
| Nambala ya Catalog | XD90008 |
| Dzina lazogulitsa | X-Gal (5-Bromo-4-chloro-3-indolyl-beta-D-galactopyranoside) |
| CAS | 7240-90-6 |
| Molecular Formula | C14H15BrClNO6 |
| Kulemera kwa Maselo | 408.63 |
| Zambiri Zosungira | -2 mpaka -6 ° C |
| Harmonize Tariff Code | 29400000 |
Mafotokozedwe a Zamalonda
| Mawonekedwe a Solution | Njira yowoneka bwino, yopanda utoto mpaka yachikasu (50mg/ml mu DMF:MeOH, 1:1) |
| Kuzungulira kwapadera kwa kuwala | -61.5 +/- 1 |
| Maonekedwe | Ufa wa crystalline woyera mpaka woyera |
| Purity HPLC | mphindi 99% |
| Kusungunuka (5 % mu DMF) | Zosungunuka (5% w/v,DMF) |
| Madzi KF | kuposa 1% |
| Kuyesa (HPLC pa Anhydrous Basis) | mphindi 98% w/w |
Kugwiritsa ntchito X-gal
X-gal (yofupikitsidwanso BCIG ya 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside) ndi organic compound yopangidwa ndi galactose yolumikizidwa ndi indole yolowa m'malo.Pagululi adapangidwa ndi Jerome Horwitz ndi othandizira mu 1964. Dzina lamankhwala lokhazikika nthawi zambiri limafupikitsidwa kuti likhale losalondola komanso liwu losavuta kwambiri monga bromochloroindoxyl galactoside.X yochokera ku indoxyl ikhoza kukhala gwero la X pakudumpha kwa X-gal.X-gal amagwiritsidwa ntchito mu biology ya ma molekyulu kuyesa kukhalapo kwa enzyme, β-galactosidase, m'malo mwazomwe amawunikira, β-galactoside.Amagwiritsidwanso ntchito kuzindikira zochita za enzyme iyi mu histochemistry ndi bacteriology.X-gal ndi amodzi mwa ma indoxyl glycosides ndi esters omwe amatulutsa zinthu zosasungunuka za buluu zomwe zimafanana ndi utoto wa indigo chifukwa cha enzyme-catalyzed hydrolysis.
X-gal ndi analogue ya lactose, motero imatha kupangidwa ndi hydrolyzed ndi enzyme ya β-galactosidase yomwe imadula chomangira cha β-glycosidic mu D-lactose.X-gal, ikadulidwa ndi β-galactosidase, imatulutsa galactose ndi 5-bromo- 4-chloro-3-hydroxyindole - 1. Yotsirizirayo imadzipangitsa kuti iwonongeke ndipo imapangidwa ndi okosijeni kukhala 5,5'-dibromo-4,4'-dichloro -indigo - 2, chinthu cha buluu kwambiri chomwe sichisungunuka.X-gal yokha ilibe mtundu, kotero kupezeka kwa zinthu zamtundu wa buluu kumatha kugwiritsidwa ntchito ngati kuyesa kukhalapo kwa β-galactosidase yogwira.Izi zimalolanso kuti bakiteriya β-galactosidase (yotchedwa lacZ ) agwiritsidwe ntchito ngati mtolankhani m'machitidwe osiyanasiyana.
Pakuwunika kwamitundu iwiri, β-galactosidase angagwiritsidwe ntchito ngati mtolankhani kuti azindikire mapuloteni omwe amalumikizana.Mwanjira iyi, malaibulale amtundu wa genome amatha kuyang'aniridwa kuti azitha kulumikizana ndi mapuloteni pogwiritsa ntchito yisiti kapena mabakiteriya.Kumene kuli kuyanjana kopambana pakati pa mapuloteni omwe akuwunikiridwa, zimabweretsa kumangiriza kwa domain activation kwa wolimbikitsa.Ngati wolimbikitsayo akugwirizana ndi jini ya lacZ, kupanga kwa β-galactosidase, komwe kumapangitsa kuti mapangidwe amtundu wa blue-pigmented pamaso pa X-gal, asonyeze kugwirizana bwino pakati pa mapuloteni.Njirayi ingakhale yongoyang'ana m'malaibulale osakwana pafupifupi 106. Kung'ambika bwino kwa X-gal kumapangitsanso fungo loipa kwambiri chifukwa cha kusinthasintha kwa indole.
Popeza X-gal payokha ilibe mtundu, kupezeka kwa zinthu zamtundu wabuluu kumatha kugwiritsidwa ntchito ngati kuyesa kukhalapo kwa β-galactosidase yogwira.
Kuzindikirika kosavuta kwa enzyme yogwira kumapangitsa kuti jini ya βgalactosidase (jini ya lacZ) igwiritsidwe ntchito ngati jini ya mtolankhani pazogwiritsa ntchito zosiyanasiyana.
