Zogulitsa

Cysteine ​​(Cys) imatenga gawo lofunikira munjira zambiri zama cell a eukaryotic ndipo kuzindikira kwake m'maselo ndikofunikira kwambiri.Komabe, glutathione (GSH), homocysteine, N-acetyl-L-cysteine ​​​​ndi ma thiols ena amalepheretsa kwambiri kuzindikira kwa Cys.Makamaka, GSH imasokoneza kwambiri kuzindikira kwa ma Cys (30-200 μM) chifukwa cha kuchuluka kwake kwa intracellular (1-10 mM).M'ntchitoyi, makina opangira fulorosenti (HOTA) ozindikira ma Cys amaperekedwa.Kafukufukuyu ali ndi chidziwitso chabwino kwambiri komanso kusankha kokwanira pakuzindikiritsa ma Cys: ndikuwonjezera kwa 200 μM Cys, mphamvu ya fluorescence ya kafukufuku (10 μM) imakwezedwa 117-fold ndipo malire ozindikira adawerengedwa kukhala 13.47 μM, omwe ndi otsika. kuposa ma Cys ndende;kafukufuku anapezanso 30-200 μM cysteine ​​pa 1-10 mM glutathione.Chifukwa chake, kuyesa kujambula ma cell kunachitika ndi probe HOTA.Komanso, zotsatira za thiol-blocki ng ndi GSH synthesis inhibiting experiments zinatsimikizira kuti kutulutsa kwa intracellular makamaka kumachokera ku mgwirizano pakati pa Cys ndi HOTA.