Zogulitsa

Multitarget mankhwala akutsatiridwa kwambiri pofuna kuchiza matenda ovuta.Apa, tikufotokoza mapangidwe ndi kaphatikizidwe ka kalasi yatsopano ya ma shogaol-huprine hybrids, omwe amayenera kugunda mipherezero ingapo yokhudzana ndi matenda a Alzheimer's.Ma hybrids ayesedwa mu vitro chifukwa cha ntchito yawo yoletsa motsutsana ndi acetylcholinesterase yaumunthu ndi butyrylcholinesterase ndi antioxidant ntchito (ABTS +, DPPH ndi Folin-Ciocalteu assays), komanso mu maselo osasunthika a Escherichia coli chifukwa cha ntchito yawo ya Aβ42 ndi tau anti-aggregating.Komanso, kulowa muubongo wawo kwayesedwa (PAMPA-BBB assay).Ngakhale ma hybrids sali amphamvu kwambiri a AChE inhibitors kapena antioxidant monga kholo la huprine Y ndi [4] -shogaol, motsatana, amawonetsabe anticholinesterase ndi antioxidant zochita ndipo ali amphamvu kwambiri Aβ42 komanso anti-aggregating agents kuposa zosakaniza za makolo.Ponseponse, ma shogaol-huprine hybrids amatuluka ngati ochititsa chidwi muubongo omwe amatsogolera anti-Alzheimer's.

Kutulutsa kwa polarity kosiyanasiyana kopezedwa kuchokera kuzigawo zosiyanasiyana za mbewu (mizu, tsamba, duwa ndi zipatso) za Seseli rigidum zidaphunziridwa ndi njira zosiyanasiyana zoyesera zoteteza antioxidant: DPPH ndi ABTS ntchito yowononga kwambiri, pakuchepetsa mphamvu zonse komanso kudzera muzathunthu za flavonoids ndi ma polyphenols. .Mafuta ofunikira a mbali zonse za mbewu adawonetsa zofooka za antioxidant.The inhibitory concentration range of the tests extracts, against bacteria Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus cereus, and fungus Candida albicans and Aspergillus niger was 0.01-1.50 mg/mL ndi microbicidal 300 mg/mL 0.00.Polumikizana ndi cholinesterase, mafuta onse ofunikira adakhala othandiza ngati zoletsa.Gawo lalikulu kwambiri la zoletsa motsutsana ndi anthu ndi mahatchi a cholinesterase adawonetsedwa ndi mizu yofunikira yamafuta (38.20% ndi 48.30%, motsatana) pakati pamafuta, ndi kuchotsa mizu ya hexane (40.56% ndi 50.65% motsatana).Mafuta ofunikira ndi zigawo zosasunthika za ziwalo zonse za zomera zidadziwika ndi GC, GC-MS ndi headspace/GC-MS.Kusanthula kwatsatanetsatane kwazotsatira kunawonetsa kuti muzu wamafuta ofunikira amasiyana kwambiri ndi mafuta ofunikira a mbali zina za mbewu.Poganizira zochitika zonse zomwe zaphunziridwa, muzu wa hexane ukuwonetsa mawonekedwe abwino kwambiri.Pogwiritsa ntchito chromatography yamadzimadzi yogwira ntchito kwambiri komanso mawonekedwe apamwamba kwambiri, zigawo 30 zochulukira kwambiri zidazindikirika m'magawo osiyanasiyana a polarity.Kukhalapo kwa zigawo zomwe zazindikirika zinali zogwirizana ndi zochitika zenizeni zamoyo, motero kupanga magulu omwe angakhale ndi udindo pazochitika zilizonse zomwe zikuwonetsedwa.