Zogulitsa

Kafukufuku wapano akufotokoza njira yosavuta komanso yabwino yogwiritsira ntchito high performance liquid chromatography (HPLC) yophatikizidwa ndi kuzindikira kwa fluorescence kuti adziwe magawo a kinetic a glutamate mu minofu yamanjenje.Minofu ya retina yomwe inapezedwa kuchokera ku anapiye amasiku 7 idakulungidwa ndi zodziwika bwino za glutamate (50-2000 μM) kwa mphindi 10, ndipo milingo ya o-phtaldehyde (OPA) -derivatized neurotransmitter mu incubation sing'anga idayesedwa.Poyesa kusiyana pakati pa zoyamba ndi zomaliza za glutamate mkatikati, njira yogwiritsira ntchito saturable inali yodziwika (K (m) = 8.2 ndi V (max) = 9.8 nmol / mg mapuloteni / min).Izi makamaka zinali zotengera sodium- ndi kutentha, kuchirikiza mwamphamvu kuti njira yochepetsera ndende imatengedwa ndi onyamula okwera kwambiri.Kuphatikiza pa izi, zotsatira zathu zidawonetsanso kuti zinc chloride (inhibitor ya glutamate / aspartate transporters) idapangitsa kuchepa kwapang'onopang'ono kwa glutama te uptake, kuwonetsa kutsimikizika kwa njira yathu.Ponseponse, ntchitoyi ili ndi njira ina yowunikira momwe ma glutamate amatengera minofu yamanjenje pogwiritsa ntchito HPLC.Njirayi ikhoza kukhala chida chofunikira pamaphunziro okhudzana ndi mawonekedwe a kusintha kwa mphindi mumayendedwe a glutamate okhudzana ndi kuvulala kwapakati pamitsempha.