Zogulitsa

Biosensor ya microRNA (miRNA) yomwe imagwiritsa ntchito ruthenium oxide nanoparticle (RuO(2) NP) -inayambitsa polymerization ya 3,3'-dimethoxybenzidine (DB) ndi miRNA-templated deposition of insulating poly(3,3'-dimethoxybenzidine) (PDB) filimu ikufotokozedwa mu ntchitoyi.The biosensor inapangidwa ndi monolayer wosakanikirana wa oligonucleotide capture probes (CPs) ndi 4-mercaptoaniline pa electrode ya golide.Kutsatira kusakanizidwa ndi RuO(2) NP-tagged target miRNA, chisakanizo cha DB/H(2)O(2) mu pH 5.0 0.10 M acetate buffer chinayikidwa pa biosensor.The RuO(2) NPs ntchito polymerization kuyambitsa/chothandizira polymerization wa DB.Ndipo zingwe zosakanizidwa za anionic miRNA ndi ma CP aulere amakhala ngati ma template, kuwongolera kuyika kwa PDB.Kuchuluka kwa PDB yoyikidwa ndi mphamvu yake yotsekera imagwirizana mwachindunji ndi kuchuluka kwa chandamale cha miRNA mu yankho.Mayeso a Electrochemical impedance spectroscopic adawonetsa kuti kulumikizana kwa liniya-kusamutsa kukana-concentration ubale kuchokera ku 6 .0 fM mpaka 2.0 pM kudapezedwa pambuyo pa mphindi 60 za incubation mu DB/H(2)O(2) osakaniza.Panalibe kusakanikirana kosakanikirana pakati pa pre-miRNA ndi miRNA yokhwima komanso kusakanizidwa kochepa kwambiri pakati pa achibale a miRNA ogwirizana ngakhale pamlingo umodzi wosiyana-siyana.Biosensor yochokera ku impedance iyi imapereka njira ina yowoneka bwino yofotokozera mbiri ya miRNA ndipo itha kupangitsa kuti pakhale mawonekedwe amtundu wa miRNA wonyamulika.