ONPG CAS:369-07-3 98.0% Min White Kupita Kuchoka -Ufa Woyera
| Nambala ya Catalog | XD90006 |
| CAS | 369-07-3 |
| Dzina lazogulitsa | ONPG(2-Nitrophenyl-beta-D-galactopyranoside) |
| Molecular Formula | C12H15NO8 |
| Kulemera kwa Maselo | 301.25 |
| Zambiri Zosungira | 2 mpaka 8 ° C |
| Harmonize Tariff Code | 29400000 |
Mafotokozedwe a Zamalonda
| Purity (HPLC) | Min.98.0% |
| Maonekedwe | White to off - woyera ufa |
| Yankho(1% m'madzi) | Njira yoyera, yopanda mtundu mpaka yachikasu pang'ono |
| M'madzi(Karl Fisher) | Max.0.5% |
| Kuzungulira kwapang'onopang'ono [α]D20(c=1, H2O) | -65.0 ° C mpaka -73.0 ° C |
Zokambirana pa mayeso a ONPG (mayeso a β-galactosidase)
Mafunso akhala akufunsidwa pafupipafupi posachedwapa: 1. Chifukwa chiyani mayeso a ONPG angagwiritsidwe ntchito kusiyanitsa kuchedwetsa kuwira kwa lactose?2. Chifukwa chiyani mulingo wadziko lonse umanena kuti ndikofunikira kugwiritsa ntchito 3% sodium chloride trisaccharide iron (kapena chitsulo cha trisaccharide) pakuyesa kwa ONPG?3. Kwa Vibrio parahaemolyticus, poyesa OPNG, chifukwa chiyani toluene iyenera kuwonjezeredwa kutsika molingana ndi muyezo?Ntchito yake ndi yotani?
Kampani yathu yawunika zambiri, kuzifotokoza mwachidule, ndikugawana nanu pansipa:
Mfundo yofunika: Dzina lachi China la ONPG ndi o-nitrobenzene-β-D-galactopyranoside.ONPG imatha kupangidwa ndi hydrolyzed kukhala galactose ndi yellow o-nitrophenol (ONP) ndi β-galactosidase, kotero ntchito ya β-galactosidase imatha kuzindikirika ndi kusintha kwamtundu wa sing'anga.
Lactose ndi shuga yemwe tizilombo tambiri timafunikira kuzindikira.Kagayidwe kake kumafuna michere iwiri, imodzi ndi cell permease, lactose imalowa m'maselo pansi pakuchita kwa permease;ina ndi β-galactosidase, yomwe imasinthira lactose kukhala galactose.Lactose ndi glucose.β-Galactosidase imathanso kuchitapo kanthu mwachindunji pa ONPG kuti ikhale hydrolyze kukhala galactose ndi yellow o-nitrophenol (ONP).Zitha kuchitika mu 24h, ngakhale ndi lactose yochedwa fermenters.Choncho, limafotokoza zotsatira zowonera za kunyamula chikhalidwe 1 kuchokera ku agar slant ndikuyiyika mozungulira pa 36 ° C kwa 1-3h ndi 24h.Ngati β-galactosidase imapangidwa, imakhala yachikasu mu 1-3h, ngati palibe enzyme yoteroyo, sisintha mtundu mu 24h.
Malinga ndi ma enzyme awiri omwe ali pamwambapa, tizilombo tating'onoting'ono titha kugawidwa m'magulu otsatirawa:
1 lactose-fermenting (maola 18-24) mabakiteriya okhala ndi permease ndi β-galactosidase P + G +;
2 Kuchedwa kwa lactose fermenters (kumatenga nthawi yopitilira maola 24) osakwanira koma kukhala ndi galactosidase: P- G+.
3 Non-lactose fermenters opanda permease ndi galactosidase: P- G-.
Mayeso a ONPG atha kugwiritsidwa ntchito kusiyanitsa mabakiteriya omwe ali ndi lactose-lag-fermenting (P-G+) ndi mabakiteriya osayatsa a lactose (PG-), monga:
1 Siyanitsani zowotchera mochedwa lactose (P- G+) ndi zofufumitsa zomwe sizikhala ndi lactose (P- G-).
(a) Citrobacter (+) ndi Salmonella arizonae (+) kuchokera ku Salmonella (-).
(b) Escherichia coli (+) wochokera kwa Shigella sonnei (-).
Chifukwa chiyani kuyesa kwa ONPG kunachitika pogwiritsa ntchito chikhalidwe chausiku pa 3% sodium chloride ferric trisaccharide (iron trisaccharide)?Kampani yathu yafunsira zambiri, koma palibe mawu omveka bwino.Pokhapokha pa webusaiti ya FDA, palembedwa kuti "Inoculate zikhalidwe kuti ziyesedwe pa katatu shuga iron agar slants ndi kuyaka kwa 18 h pa 37 ° C (kapena kutentha kwina koyenera, ngati kuli kofunikira) . Zopatsa thanzi (kapena zina) agar slants okhala ndi 1.0 % lactose ingagwiritsidwenso ntchito.amatanthauza: mabakiteriya oyesedwa adalowetsedwa pazitsulo zachitsulo cha trisaccharide ndipo amakula pa 37 ° C kwa 18h.Zakudya za agar slants (kapena zina) sing'anga yokhala ndi 1% lactose ndizovomerezeka.Chifukwa chake, zimaganiziridwa kuti sing'anga yachitsulo ya trisaccharide ili ndi lactose.Pambuyo pakukula kwausiku, mabakiteriya apanga β-galactosidase yabwino yogwira ntchito.Pogwiritsa ntchito mabakiteriya otere, ONPG imatha kuwola ndi β-galactosidase mwachangu.The experimental chodabwitsa ndi mofulumira ndi bwino kuwonetseredwa.Kuphatikiza apo, kuwonjezera toluene ndi madzi osamba kwa mphindi 5 zonse ndicholinga chotulutsa β-galactosidase.
