nkhani

Pali ngwazi zambiri zosaimbidwa kuzungulira ife, zomwe zimawoneka ngati zamba, koma zoona zake mwakachetechete zimapereka zambiri kwa ife.Proteinase K ndi "ngwazi yosadziwika" mumakampani opanga ma molecular diagnostics, ngakhale poyerekeza ndi "wamkulu ndi amphamvu" m'makampani, proteinase K ndi yotsika kwambiri kotero kuti takhala tikunyalanyaza kufunika kwake.Ndi kufalikira kwa mliri watsopano wa korona, kufunikira kwa proteinase K kwakula, ndipo kupezeka kwanyumba ndi kunja kwatsala pang'ono kumwa, ndipo aliyense mwadzidzidzi adazindikira kuti proteinase K ndiyofunikira kwambiri.
Kodi kugwiritsa ntchito proteinase K ndi chiyani?
Proteinase K ndi serine protease yokhala ndi proteolytic enzyme ntchito ndipo imatha kusunga ntchito m'malo osiyanasiyana (pH (4-12.5), mchere wambiri, kutentha kwa 70 ° C, etc.).Kuonjezera apo, ntchito ya proteinase K sikuletsedwa ndi SDS, urea, EDTA, guanidine hydrochloride, guanidine isothiocyanate, ndi zina zotero, komanso kuchuluka kwa detergent kungapangitsenso ntchito ya proteinase K. Mu chithandizo chamankhwala (virus ndi tizilombo toyambitsa matenda tizilombo toyambitsa matenda ), chakudya (kutentha nyama), chikopa (kufewetsa tsitsi), kupanga vinyo (kumveketsa mowa), kukonzekera kwa amino acid (nthenga zowonongeka), nucleic acid m'zigawo, in situ hybridization, etc., proteinase K Pali ntchito.Ntchito yomwe imagwiritsidwa ntchito kwambiri ndikuchotsa nucleic acid.
Proteinase K imatha kupanga enzymolyze mitundu yonse ya mapuloteni muzatsanzo, kuphatikiza ma histones omwe amamangiriridwa mwamphamvu ku nucleic acid, kotero kuti ma nucleic acid amatha kutulutsidwa kuchokera pachitsanzo ndikutulutsidwa muchocho, ndikuwongolera sitepe yotsatira yochotsa ndi kuyeretsa.Pozindikira ma viral nucleic acid, proteinase K ndi imodzi mwazinthu zofunika kwambiri pakuyesa ma virus.Proteinase K imatha kusweka ndikuyambitsa puloteni ya kachilomboka, yomwe imakhala yotetezeka panthawi yamayendedwe ndi kuzindikira;Kuphatikiza apo, proteinase K imathanso kusokoneza RNase imalepheretsa kuwonongeka kwa ma virus a RNA komanso imathandizira kuzindikira kwa nucleic acid.
Kutchuka kwausiku wa proteinase K
Kaya m'munda wa kafukufuku wa sayansi kapena m'munda wa IVD, kuchotsa nucleic acid ndiko kuyesa kofunikira kwambiri, kotero proteinase K yakhala yofunikira kwambiri.Komabe, m'mbuyomu, proteinase K inali yodziwika kwambiri kuposa ntchito yake.Gawo lalikulu la izi linali chifukwa ubale wopezeka ndi kufunikira kwa proteinase K unali wokhazikika.Ndi anthu ochepa amene angaganize kuti kupezeka kwa proteinase K kungakhale vuto.
Ndi kufalikira kwa mliri watsopano wa korona, kufunikira kwa kuyesa kwa nucleic acid kwakula.Pofika kumapeto kwa Juni 2020, China yamaliza mayeso atsopano pafupifupi 90 miliyoni, ndipo chiwerengerochi ndi chowopsa kwambiri padziko lonse lapansi.Mu kuyesa kwa nucleic acid, kuchuluka kwa proteinase K ndi pafupifupi 50-200 μg/mL.Nthawi zambiri, zimatengera pafupifupi 100 μg wa proteinase K kuti atenge chitsanzo cha nucleic acid.Pogwiritsira ntchito kwenikweni, kuti muwonjezere mphamvu ya nucleic acid m'zigawo, nthawi zambiri Proteinase K idzagwiritsidwa ntchito mu kuchuluka kwachulukidwe.Kuzindikira kwa Nucleic acid kwa coronavirus yatsopano kwabweretsa kuchuluka kwa proteinase K.Kukwanira kokwanira kwa proteinase K komanso kufunikira kwa proteinase K kudasweka mwachangu, ndipo proteinase K idakhala chinthu chofunikira kwambiri chopewera miliri usiku umodzi.
Zovuta pakupanga proteinase K
Ngakhale ndi chitukuko cha mliriwu, phindu lofunika la proteinase K lakhala likudziwika ndi anthu, ndizochititsa manyazi kuti chifukwa cha makiyi otsika kwambiri a proteinase K, makampani ochepa apakhomo adagwira nawo ntchito yopanga proteinase K. Pamene anthu ndikufuna kukhazikitsa proteinase K kupanga Popanga, zidapezeka kuti proteinase K ndi puloteni yapadera kwambiri.Ndizovuta kwambiri kukulitsa mphamvu yopangira proteinase K m'kanthawi kochepa.
Kupanga kwakukulu kwa proteinase K kumakumana ndi zovuta zotsatirazi
1. Mawu otsika
Proteinase K satha kuwononga mapuloteni ambiri ndikuyambitsa kawopsedwe ku cell host host.Chifukwa chake, mulingo wa proteinase K nthawi zambiri umakhala wotsika kwambiri.Kuwunika kwa machitidwe ndi mitundu yomwe imawonetsa proteinase K nthawi zambiri imafunikira nthawi yayitali.
2. Zotsalira za inki ndi nucleic acid
Kuwotchera kwakukulu kumabweretsa kuchuluka kwa pigment ndi zotsalira za nucleic acid.Zimakhala zovuta kuchotsa zonyansazi ndi njira yosavuta yoyeretsera, ndipo kuyeretsa kovutirapo kumawonjezera mtengo ndikuchepetsa kuchira.
3. Kusakhazikika
Proteinase K sikhazikika mokwanira, imatha kupanga enzymolyze yokha, ndipo ndizovuta kuisunga mokhazikika pa 37 ° C kwa nthawi yayitali popanda woteteza.
4. Mosavuta kugwa
Pokonzekera ufa wowuma wowuma wa proteinase K, kuti muwonetsetse kuti zolimba za proteinase K mu ufa wowuma ndizazikulu, ndikofunikira kuwonjezera chitetezo chowumitsidwa pamlingo waukulu, koma ngati kuchuluka kwa proteinase K kumafika 20mg/mL ndi kupitilira apo, ndikosavuta Kuphatikizika kumapanga mpweya, zomwe zimabweretsa zovuta zazikulu pakuwumitsa kwa proteinase K yokhala ndi zolimba kwambiri.
5. Ndalama zazikulu
Proteinase K imakhala ndi mphamvu ya proteinase ndipo imatha kutulutsa ma proteinase ena mu labotale.Chifukwa chake, proteinase K imafunikira madera opangira apadera, zida, ndi ogwira ntchito pa kafukufuku ndi chitukuko ndi kupanga.
XD BIOCHEM ya proteinase K yankho
XD BIOCHEM has a mature protein expression and purification platform, and has rich experience in the expression and purification of recombinant proteins and optimization of production processes. Through the rapid formation of a research and development team, the large-scale production process of proteinase K has been overcome. The monthly output of freeze-dried powder is more than 30 KG. The product has stable performance, high enzyme specific activity, and no host cytochrome and nucleic acid residues. Welcome to contact XD BIOCHEM Obtain a trial package (E-mail: sales@xdbiochem.com Tel: +86 513 81163739).
Mayankho aukadaulo a XD BIOCHEM akuphatikiza
Pogwiritsa ntchito kuphatikizika kwa plasmid yamitundu yambiri, zovuta zowonetsera kwambiri zomwe zili ndi 8g / L zimasankhidwa, zomwe zimagonjetsa vuto la kuchepa kwa proteinase K;
Kupyolera mu kukhazikitsidwa kwa njira yoyeretsera masitepe ambiri, zotsalira za cytochrome ndi nucleic acid za proteinase K zinachotsedwa bwino pansi pa mtengo wamba;
Kupyolera mu kuwunika kwapamwamba kwa mapangidwe a chitetezo cha chitetezo, chotchinga chomwe chingathe kusunga proteinase K pa 37 ° C chinasankhidwa;
Zosungira zowunikira zimathana ndi vuto loti proteinase K ndiyosavuta kusonkhanitsa ndi kugwa pang'onopang'ono, ndipo imayala maziko a proteinase K's high solid content kuunika-kuzizira.

XD BIOCHEM proteinase K chitsanzo

Mayeso okhazikika a XD BIOCHEM proteinase K: sipadzakhala kusintha kwakukulu muzochitika pambuyo pa 80 d kutentha

Mayeso okhazikika a XD BIOCHEM proteinase K: sipadzakhala kusintha kwakukulu muzochitika pambuyo pa 80 d kutentha

Kuyerekeza kwa nucleic acid m'zigawo za XD BIOCHEM proteinase K ndi zinthu zopikisana.Pochotsa nucleic acid, XD BIOCHEM ndi mpikisano wa proteinase K amagwiritsidwa ntchito motsatana.Kuchita bwino kwa XD BIOCHEM proteinase K ndikokwera kwambiri ndipo mtengo wa Ct wa jini yomwe mukufuna ndi yotsika.