Zogulitsa

Kuopsa kwa kukana kwa tizilombo toyambitsa matenda pamodzi ndi kusowa kwa mankhwala atsopano oletsa tizilombo toyambitsa matenda kwawonjezera chidwi pa chitukuko cha antimicrobial peptides (AMPs) monga mankhwala atsopano.Mu kafukufukuyu, tidawunika zochita za antimicrobial za peptides ziwiri zazifupi zopanga, zomwe ndi, RRIKA ndi RR.Ma peptideswa adawonetsa zochita zamphamvu za antimicrobial motsutsana ndi Staphylococcus aureus, ndipo zotsatira zake zowononga tizilombo zidakulitsidwa kwambiri powonjezera ma amino acid atatu mu C terminus, zomwe zidapangitsa kuti amphipathicity, hydrophobicity, and net charge.Komanso, RRIKA ndi RR zinasonyeza mphamvu yaikulu komanso yofulumira ya bactericidal polimbana ndi matenda a Staphylococcus osamva mankhwala, kuphatikizapo methicillin-resistant Staphylococcus aureus (MRSA), vancomycin-intermediate S. aureus (VISA), vancomycin-resistant S. aureus (VRSA) , S. aureus yosamva linezolid, ndi Staphylococcus epidermidis yolimbana ndi methicillin.Mosiyana ndi ma AMP ambiri achilengedwe, RRIKA ndi RR adasunga ntchito zolowa m'malo mwa NaCl ndi MgCl2.Onse a RRIKA ndi RR adalimbikitsa kuphedwa kwa lysostaphin mopitilira 1,000 ndikuchotsa kudzipatula kwa MRSA ndi VRSA mkati mwa mphindi 20.Kuphatikiza apo, ma peptide omwe adaperekedwa anali apamwamba kwambiri pakuchepetsa ma biofilms ogwirizana a S. aureus ndi S. epidermidis poyerekeza ndi zotsatira ndi maantibayotiki wamba.Zomwe tapeza zikuwonetsa kuti staphylocidal zotsatira za ma peptides athu zidachitika chifukwa cha kufalikira kwa nembanemba ya bakiteriya, zomwe zimapangitsa kuti ma cytoplasmic atayike komanso kufa kwa cell.Komanso, ma peptides sanali poizoni kwa maselo a HeLa pa 4- kwa 8-kuchulukitsa kwawo kwa antimicrobial.Mankhwala amphamvu komanso osamva mchere a ma peptidewa amapereka chithandizo chowoneka bwino chochiza matenda osagwirizana ndi mankhwala a S. aureus.