L-Leucine Cas: 61-90-5 99% ufa woyera
| Nambala ya Catalog | XD90304 |
| Dzina lazogulitsa | L-Leucine |
| CAS | 61-90-5 |
| Molecular Formula | C6H13NO2 |
| Kulemera kwa Maselo | 131.17292 |
| Zambiri Zosungira | Wozungulira |
| Harmonize Tariff Code | 29224985 |
Mafotokozedwe a Zamalonda
Mofanana ndi ma virus ena a RNA, ma tombusvirus amapangidwanso ndi membrane-bound viral replicase complex (VRC).VRC imakhala ndi p92 virus-coded RNA polymerase (RdRp), viral p33 RNA chaperone, ndi mapuloteni angapo omwe amasankhidwa nawo.Kuti ikhale RdRp yogwira ntchito pambuyo pomasulira, puloteni yobwerezabwereza ya p92 iyenera kuphatikizidwa mu VRC, ndikutsatiridwa ndi kuyambitsa kwake.Tawonetsa m'mbuyomu mu kuyesa kopanda yisiti kopanda ma cell kuti kuyambitsa kwa Tomato bushy stunt virus (TBSV) RdRp kumafuna zosungunuka zosungirako zinthu.M'nkhaniyi, tikuzindikira puloteni yochititsa chidwi ya ma cell 70 (Hsp70) ngati chinthu chosankhidwa chothandizira kuti atsegule N-terminally truncated recombinant TBSV RdRp.Kuphatikiza apo, kutsekeka kwa mamolekyu ang'onoang'ono a Hsp70 kumalepheretsa kaphatikizidwe ka RNA ndi tombusvirus RdRp mu vitro.Kuphatikiza apo, tikuwonetsa kuti ma phospholipids osalowerera ndale, omwe ndi phosphatidylethanolamine (PE) ndi phosphatidylcholine (PC), amathandizira kuyambitsa kwa RdRp mu vitro.Mosiyana ndi izi, phosphatidylglycerol (PG) ikuwonetsa mphamvu yoletsa komanso yoletsa kuyambitsa mu vitro RdRp.Tikuwonetsanso kuti PE ndi PC zimathandizira kulumikizana kwa RdRp-viral plus-strand RNA [(+)RNA], pomwe PG imaletsa kumangirira kwa ma virus a RNA ku RdRp.Kutengera ndi ntchito zoletsa zoletsa za phospholipids mu tombusvirus RdRp activation, tikuganiza kuti kapangidwe ka lipid kamene kamayang'aniridwa ndi nembanemba atha kugwiritsidwa ntchito ndi ma tombusvirus kuwongolera msonkhano watsopano wa VRC panthawi ya matenda. (RdRp), yomwe imayang'anira kupanga ma virus a RNA m'maselo omwe ali ndi ma virus angapo a RNA, imasiya kugwira ntchito.Njirayi ikuyenera kupeŵa kaphatikizidwe ka ma virus a RNA mu cytosol yomwe ingayambitse mwachangu mayankho a RNA oyambitsa ma cellular antiviral.Pakuphatikiza kwa membrane-bound replicase complex, viral RdRp imayamba kulumikizidwa kudzera munjira yosamvetsetseka yomwe imapangitsa RdRp kukhala yokhoza kupanga RNA.Pogwiritsa ntchito TBSV RdRp, tikuwonetsa kuti ma cell a Hsp70 chaperone ndi ma phospholipids osalowerera ndale amathandizira RdRp activation in vitro.Mosiyana ndi izi, phosphatidylglycerol (PG) imakhala ndi mphamvu yoletsa kutsegulira kwa in vitro RdRp ndi kuyanjana kwa RdRp-viral RNA, kutanthauza kuti membranous microdomain yozungulira RdRp imakhudza kwambiri kuthekera kwake kwa kaphatikizidwe ka RNA.Chifukwa chake, kuyambitsa kwa viral RdRp mwina kumadalira zigawo zingapo zamagulu omwe ali ndi kachilomboka.
4-Nitrophenyl-N-acetyl-β- D-glucosaminide ndi gawo lothandiza lachidziwitso chofulumira cha colorimetric cha ntchito ya N-acetyl-b-glucosaminidase mu mkodzo waumunthu.Chromogenic β-Glucosaminidase gawo lapansi lomwe limatulutsa chikasu pa cleavage, lomwe limagwiritsidwa ntchito makamaka mu yisiti ndi nkhungu.
