Zogulitsa

Choyambitsa chachikulu cha matenda a Clostridium difficile (CDI) ndi kugwiritsa ntchito maantibayotiki chifukwa chosokonekera kwa mgwirizano wa m'matumbo a m'matumbo.Kusunga mabakiteriya opindulitsa okhala ndi ma probiotic panthawi ya chithandizo cha matenda, kugwiritsa ntchito mamolekyu okhala ndi antibacterial yosankha kumawonjezera mphamvu pakuchotsa C. difficile.Mmodzi wa iwo ndi chomera alkaloid 8-hydroxyquinoline (8HQ), amene wasonyeza kusankha ziletsa clostridia popanda repressing bifidobacteria.Ntchito yosankha antimicrobial nthawi zambiri imayesedwa ndi chikhalidwe cha mitundu ya bakiteriya.Komabe, cholepheretsa chachikulu cha njirazi ndikulephera kufotokoza kusiyana kwa kukula kwa mitundu yambiri ya mabakiteriya mu co-culture mkati mwa kuyesa komweko.Pakafukufuku wamakono, tinagwirizanitsa fulorosenti mu situ hybridization ndi kutuluka kwa cytometry kuti tifotokoze kusintha kwa maselo ogwira ntchito komanso osagwira ntchito a chikhalidwe chosakanikirana chopangidwa ndi tizilombo toyambitsa matenda C. diff icile CECT 531 ndi phindu la Bifidobacterium longum subsp.longum CCMDMND BL1 pambuyo kukhudzana ndi 8HQ.Zinawonetsedwa kuti popanda 8HQ, chiwerengero cha zovuta zonsezi chinali pafupifupi chofanana, chozungulira pakati pa 22.7 ndi 77.9% panthawi ya 12 h, pamene ndi 8HQ chiwerengero cha C. difficile yogwira ntchito chinachepa pambuyo pa 4 h, ndipo chinapitirira pakati pa 8.8 yokha. ndi 17.5%.Mosiyana ndi izi, kukula kwa bifidobacterial sikunasokonezedwe ndi 8HQ.Zotsatira za phunziroli zinawonetsa zotsatira zoletsa za 8HQ pa clostridial ndi bifidobacterial kukula mphamvu, ndi kuthekera kwa pawiri pa chitukuko cha osankhidwa kuti azilamulira ma CDI.