Zogulitsa

Magulu a iron-sulfure amagwira ntchito ngati ma cofactors a mapuloteni osiyanasiyana, okhala ndi magawo osiyanasiyana a ma cell mu ma cell a prokaryotic ndi eukaryotic.Makina odzipatulira amasonkhanitsa masango ndikuwapereka ku mamolekyu omaliza omwe amavomereza mokhazikika.Mu prototypical Gram-negative bacteria Escherichia coli, njira ziwiri zomwe zilipo kale za iron-sulfur cluster, iron-sulfur cluster (ISC) ndi sulfur assimilation (SUF) njira, ndizolumikizana kwambiri.The ISC pathway regulator, IscR, ndi cholembera chamtundu wa helix-turn-helix chomwe chingathe kugwirizanitsa gulu la [2Fe-2S].Kupezeka kwa redox komanso kupezeka kwachitsulo kapena sulfure kumapangitsa kuti gulu la labile la IscR lisinthe, lomwe limatsimikizira kusintha kwa DNA kwa owongolera: IscR yokhala ndi cluster imatha kumangirira kubanja la otsatsa ma gene (mtundu-1) pomwe mawonekedwe opanda clusterless amazindikira gulu lachiwiri lotsatizana (mtundu-2).Komabe, biogenesis ya iron-sulfur cluster biogenesis mu mabakiteriya a Gram-positi sadziwika bwino, ndipo zamoyo zambiri za gululi zimawonetsa gulu limodzi lokha lamagulu amagulu a iron-sulfure.Chosiyana kwambiri ndi chitsulo chochepetsera mabakiteriya a Gram-positive Thermincola potens, pomwe majini a machitidwe onsewa amatha kudziwika, ngakhale ndi gulu lopatuka kuchokera ku mabakiteriya a Gram-negative.Tidawonetsa kuti imodzi mwa jiniyi imakhala ndi ma homolog ogwirira ntchito a IscR ndipo zikutheka kuti ikukhudzidwa ndi kayendetsedwe ka iron-sulfur cluster biogenesis mu T. potens.Mawonekedwe a T. potens ndi E. coli IscR adavumbulutsa kamangidwe kofananako ndipo adavumbulutsa kusamalidwa kosayembekezereka kwa njira yapadera yatsankho lotsatizana la gulu losiyana ili la owongolera zolembera.