Zogulitsa

Kukalamba kwanthawi yama cell a yisiti nthawi zambiri amagwiritsidwa ntchito ngati chitsanzo cha ukalamba wa maselo a post-mitotic.Yisiti ya Saccharomyces cerevisiae yomwe imamera pa glucose pamaso pa ammonium sulphate imagwiritsidwa ntchito kwambiri pofufuza zaukalamba wa yisiti.Tasanthula nthawi yokalamba ya yisiti ya Hansenula polymorpha yomwe imakula pazomwe zimafunikira kagayidwe kake ka peroxisome kuti ikule. izo sizifuna peroxisomes.Ufupi wa moyo wa H. polymorpha pa shuga makamaka chifukwa cha acidification yapakatikati, pomwe nthawi zambiri ROS sikhala ndi gawo lofunikira.Kukula kwa maselo pa methanol/methylamine m'malo mwa methanol/ammonium sulphate kunapangitsa kuti moyo ukhale wowonjezereka.Izi zinali zosagwirizana ndi acidization yapakati.Tikuwonetsa kuti oxidation ya methylamine ndi peroxisomal amine oxidase pamikhalidwe ya njala ya kaboni ndiyothandiza pakukulitsa moyo.The methylamine oxidation product formaldehyde imapangidwanso ndi okosijeni zomwe zimapangitsa kuti NADH ipangidwe, zomwe zimathandiza kuti kuwonjezeka kwa ATP ndi kuchepetsa milingo ya ROS mu gawo lokhazikika.Kuphatikiza apo, kuthekera kopanga NADH panjala ya kaboni ndi gwero la nayitrogeni kumathandizira kukulitsa moyo wa cell.Chifukwa chake, kutanthauzira kwa CLS kusanthula mu yisiti kuyenera kukhala ndi zotsatira zomwe zingatheke pa mphamvu ya cell.