Zogulitsa

Chimodzi mwazinthu zomwe zimalepheretsa kugwiritsa ntchito mankhwala obwezeretsanso ku matenda osokonekera a cartilage ndi magwero abwino a maselo.Chondrocyte, mtundu wokhawo wamtundu wa cartilage, womwe umakula mu vitro pansi pa chikhalidwe kuti uwonjezere ma cell cell amataya phenotype yawo komanso kuthekera kopanga minofu ya hyaline cartilaginous.Mu phunziro ili timazindikira kuti serum- ndi kukula-free factor-free-three-dimensional (3D) chikhalidwe dongosolo limabwezeretsanso mphamvu ya chondrocyte yodutsa kupanga minofu ya cartilage mu vitro, ndondomeko yomwe imaphatikizapo sox9.Bovine articular chondrocytes anadutsa kawiri kuti kulola kukulitsidwa kwa ma cell (P2) ndikukulitsidwa kwambiri pazingwe za 3D collagen-type-II-zokutidwa ndi glucose wambiri wophatikizidwa ndi insulin ndi dexamethasone (SF3D).Maselowo adadziwika pambuyo pakukula kwa monolayer ndikutsata chikhalidwe cha 3D ndi flow cytometry, gene expression, ndi histology.Kusintha koyambirira kwa njira zowonetsera zizindikiro panthawi ya redifferentiat ion zinali zodziwika.Maselo a P2 amasonyeza progenitor-ngati antigen profile ya 99% CD44 (+) ndi 40% CD105 (+) ndi jini yowonetsera ma cell a interzone.P2 mu SF3D idawonetsa majini a chondrogenic komanso matrix owonjezera.Kuchepetsa insulin receptor (IR) yokhala ndi HNMPA-(AM3) kapena PI-3/AKT kinase pathway (yoyendetsedwa ndi chithandizo cha insulin) ndi Wortmannin inhibited collagen synthesis.HNMPA-(AM3) yachepetsa mawu a Col2, Col11, ndi majini a IR komanso Sox6 ndi -9.Co-immunoprecipitation ndi chromatin immunoprecipitation analysis of HNMPA-(AM3) -cellated cells anasonyeza kumangirira kwa coactivators Sox6 ndi Med12 ndi Sox9 koma kuchepetsa Sox9-Col2a1 kumanga. kupangidwa kwa minofu ya cartilage ya hyaline mwa gawo limodzi ndi kumanga kwa insulin-mediated Sox9-Col2a1.Kuyenerera kwa minofu yopangidwa kudzera m'njira imeneyi kuti igwiritsidwe ntchito pokonzanso pamodzi kuyenera kuwunikiridwa mu vivo.