Zogulitsa

Kafirin ndi puloteni yachilengedwe, ya hydrophobic ndi celiac yotetezeka ya prolamin yotengedwa ku njere za manyuchi.Masiku ano kafirin imapezeka kuti ndi yothandiza popanga njira zochedwa zoperekera ndi zokutira za mankhwala ndi zakudya zopatsa thanzi pomwe chiyero chake ndi chofunikira ndipo izi zitha kupezeka ndi adsorptive chromatography.Kafukufukuyu ndi chidziwitso choyamba cha sayansi pa isotherm ndi maphunziro a kinetic a kafirin adsorption pa anion- ndi cation-exchange resins kuti agwiritse ntchito pa preparative scale chromatography.Adsorption isotherms ya kafirin idatsimikiziridwa kuti ma anion asanu- ndi ma resin awiri a cation-exchange-exchange resin mu batch system.Magawo a Isotherm monga kuthekera kokwanira komangiriza ndi kulekanitsa kosalekeza adatsimikiziridwa kuchokera ku Langmuir isotherm, ndi mphamvu ya adsorptive ndi kuyanjana kosalekeza kuchokera ku Freundlich isotherm.Langmuir isotherm idapezeka kuti ikugwirizana bwino ndi data ya adsorption.Ma kinetics a batch uptake for kafirin adsorption pa resins izi zidachitikanso ndipo magawo ofunikira kuphatikiza kufalikira kwa coefficient, coefficient ya kutengera filimu, ndi nambala ya Biot ya mtundu wa filimu-pore diffusion idawerengedwa.Zonse za isotherm ndi kinetic zinaganiziridwa kuti zisankhe utomoni woyenera woyeretsa kafirin.UNOsphere Q (78.26 mg/ml) ndi Toyopearl SP-650M (57.4 mg/ml) anapezeka kuti amapereka mphamvu zomangirira za kafirin komanso mphamvu yolumikizirana ndi kinetics yabwino kwambiri yotengera pansi pazikhalidwe zogwirira ntchito.Ndi ma adsorbents awa, kukana kufalikira kwa filimu kunapezeka kuti ndizofunikira kwambiri pakutsatsa (Bi<10 ndi δ<1).Kutengera ndi cholinga cha wopanga, UNOsphere Q idapezeka kuti ndi yabwino kwambiri yomangirira kafirin.Zomwe zaperekedwa ndizofunika kwambiri popanga makina akuluakulu okonzekera adsorptive chromatographic kafirin purification