Zogulitsa

Tinapanga kachitidwe kakang'ono kosokoneza ka RNA (siRNA) pogwiritsa ntchito ternary complex yokhala ndi polyethyleneimine (PEI) ndi γ-polyglutamic acid (γ-PGA), yomwe imasonyeza kutonthola komanso kusakhala ndi cytotoxicity.Mafakitale a siRNA okhala ndi PEI anali pafupifupi 73-102 nm mu kukula kwa tinthu ndi 45-52 mV mu ζ-kuthekera.Kutonthola kwa ma siRNA / PEI complexes kunawonjezeka ndi kuwonjezeka kwa PEI, ndipo ma siRNA / PEI maofesi omwe ali ndi chiŵerengero cha malipiro oposa 16 amasonyeza kugogoda kwa luciferase mu mbewa colon carcinoma cell line yomwe imasonyeza luciferase (maselo a Colon26 / Luc).Komabe, cytotoxicity yamphamvu ndi kuphatikizika kwa magazi kunawonedwa mu siRNA/Lipofectamine complex ndi siRNA/PEI16.Kubwezeretsanso ma cationic complexes ndi anionic compound kunanenedwa kuti ndi njira yodalirika yothetsera izi.Chifukwa chake tidakonza ma ternary complexes a siRNA okhala ndi PEI (charge ratio 16) powonjezera γ-PGA kuti achepetse cytotoxicity ndikupereka siRNA.Monga momwe zimayembekezeredwa, cytotoxicity ya ternary complexes inatsika ndi kuwonjezeka kwa γ-PGA, zomwe zinachepetsa ζ-kuthekera kwa zovutazo.Kutonthola kwamphamvu kofanana ndi siRNA/Lipofectamine complex kunapezeka m'mabwalo apakati kuphatikiza γ-PGA okhala ndi anionic surface charger.Kuphatikizika kwakukulu kwa ma ternary complexes m'maselo a Colon26/Luc kunatsimikiziridwa ndi microcopy ya fluorescence.Atakwaniritsa kugwetsa kwa jini yomwe idasinthidwa modabwitsa, kuthekera kwa zovutazo kuti zithandizire kugwetsa kwa jini yosunga nyumba, glyceraldehyde 3-phosphate dehydrogenase (GAPDH), idawunikidwa m'maselo a B16-F10.The ternary complex (siRNA/PEI16/γ-PGA12 complex) inawonetsa kwambiri GAPDH knockdown effect.Chifukwa chake, tinapanga njira yothandiza yoperekera siRNA.