Zogulitsa

Spiral tube assembly counter-current chromatography idagwiritsidwa ntchito bwino pakupatula dl-tryptophan pogwiritsa ntchito bovine serum albumin ngati chosankha cha chiral.Dongosolo labwino la biphasic aqueous-aqueous solvent system 12.0% (w/w) polyethyleneglycol 8000-9.0% (w/w) dibasic potaziyamu phosphate-0.1% ammonia-78.9% madzi adagwiritsidwa ntchito ngati njira yosungunulira ya chromatography yapano, momwe seramu ya bovine albumin idagawidwa kwambiri m'gawo lotsika la magawo awiri amadzimadzi.Dongosolo la zosungunulira zamadzimadzi lamadzimadzi linapatsa enantioselectivity yapamwamba kwambiri ya d- ndi l-tryptophan pa α=2.605 pamodzi ndi chiŵerengero chogawa DD=1.200 ndi DL=0.461.Kusamvana kwapamwamba kwambiri kunapezedwa pofuna kusiyanitsa 2.0mg ya dl-tryptophan ndi spiral tube assembly counter-current chromatography pansi pa kutentha kwa chipinda.Zinapezeka kuti 0.1% ammonia yowonjezeredwa mumadzi osungunulira amadzimadzi amadzimadzi amathandizira kwambiri ma enantioseparations.Kuchuluka kwachilendo kwakukulu kwa l-tryptophan kunawonedwa panthawi ya antioseparations.Kuti apereke kufotokozera, kuchuluka kwa kusamutsa kwa d- ndi l-enantiomers kudzera mu mawonekedwe apakati pa magawo awiriwa adayesedwa.Zinapezeka kuti l-tryptophan adawonetsa kutsika kwapang'onopang'ono kuposa d-tryptophan.Kukambitsirana kwina kunaperekedwa pazifukwa zomwe zingatheke kuti pakhale kusiyana kwa kuchuluka kwa ma enantiomers.