Zogulitsa

γ-Glutamyltranspeptidases (γ-GTs) imadula chomangira cha γ-glutamyl amide cha glutathione ndikusamutsa gulu lotulutsidwa la γ-glutamyl kumadzi (hydrolysis) kapena kulandira ma amino acid (transpeptidation).Ma enzymes omwe amapezeka paliponse amatenga gawo lalikulu pa biosynthesis ndi kuwonongeka kwa glutathione, komanso pochotsa poizoni wa xenobiotic.Apa tikuwonetsa mawonekedwe a kristalo a 3Å a Bacillus licheniformis γ-GT (BlGT) komanso zovuta zake ndi l-Glu.Mapangidwe a X-ray amatsimikizira kuti BlGT ndi ya N-terminal nucleophilic hydrolase superfamily ndipo imawulula kuti puloteniyo ili ndi malo otseguka otseguka ofanana ndi omwe amanenedwa kuti ndi homologous enzyme yochokera ku Bacillus subtilis, koma yosiyana ndi yomwe imawonedwa kwa anthu γ-GT ndi kwa γ-GTs kuchokera ku tizilombo tina.Deta ikuwonetsa kuti kumangika kwa l-Glu kumapangitsa kuyitanitsanso kwa C-terminal mchira wa BlGT subunit yayikulu ndikulola kuzindikirika kwa gulu la zotsalira za asidi zomwe zitha kuphatikizidwa pakuzindikirika kwa ayoni yachitsulo.Udindo wa zotsalirazi pa kukhazikika kwa BlGT waphunziridwa powonetsa kusintha kwa autoprocessing, enzymatic activity, chemical and thermal denaturation of four new Ala single mutants.Zotsatira zikuwonetsa kuti kusinthidwa kwa Asp568 ndi Ala kumakhudza kukhazikika kwapang'onopang'ono komanso kukhazikika kwa mapuloteni.