Zogulitsa

Adsorption of proteins to solid-fluid interfaces nthawi zambiri imapezeka kuti imalimbikitsa kupanga zosungunuka zosungunuka ndi zazikulu, zowoneka bwino, ndi tinthu tating'onoting'ono, koma magawo ofunikira munjira iyi nthawi zambiri amakhala ovuta kufufuza mwachindunji.Kuphatikizika komwe kumalumikizidwa ndi adsorption kumadzi-silicon oxide (SiOx) zolumikizira, zofanana ndi magalasi a hydrated, zidadziwika ngati ntchito ya pH ndi mphamvu ya ionic ya alpha-chymotrypsinogen (aCgn) komanso antibody monoclonal (IgG1).Selo loyenda limalola kuwunikira kwa neutron pamapuloteni omwe amawakongoletsa kuti ayeretse malo a SiOx, komanso pambuyo poti "mutsuka" motsatizana.Aggregates anapeza yankho pambuyo "kuchacha" pang'onopang'ono pamwamba ankadziwika ndi neutroni kumwazikana, maikulosikopu, ndi fluorescence spectroscopy.Mamolekyu a IgG1 omwe amangoyang'ana makamaka "lathyathyathya" kumtunda kwa SiOx, ndi gawo loyambira la mapuloteni osungunuka pang'ono, pomwe zokutira zomata zimachapidwa mosavuta.Ma aCgn mamolekyu anali osagwirizana ndi kuwonongeka pamene amawonekera kuti avumbulutsidwe pa mawonekedwe, koma amachotsedwa mosavuta.Pazochitika zomwe zimamangiriza mwamphamvu, mapuloteni omwe adachita desorb anali osakaniza a monomer ndi magawo ang'onoang'ono a HMW aggregates (ya aCgn) kapena tinthu tating'ono (ya IgG1).Kusintha kwa ma adsorption ndi / kapena kufutukuka ndi pH kumasonyeza kuti kuyanjana kwa electrostatic kunali kofunikira nthawi zonse.