Monoclonal antibody (mAb 8281) yokhudzana ndi terminal β-galactose (βGal) ya glycosphingolipids (GSL) ndi glycoproteins idapangidwa kuchokera ku mbewa zotetezedwa ndi lipid extract kuchokera ku maselo atsopano a acute lymphocytic leukemia (ALL).Immuno-thin layer chromatography (ITLC) ndi kuyesa kwa mpikisano ndi miyezo yoyeretsedwa ya GSL, shuga waulere, ndi ma neoglycoprotein opangidwa adawonetsa mAb 8281 kuti ikhale yogwira ntchito kwambiri ndi LacCer, GalCer ndi Gal-β-O-(CH3)2S(CH3)2- CONH-(Gal-β-O-CETE) yolumikizidwa ndi bovine serum albumin (BSA).Shuga wotsogola nawonso adagwira nawo ntchito yomanga.Ma antibody sanali ochitapo kanthu ndi ma carbohydrate okhala ndi ma terminal αGal mapangidwe komanso magawo osagwirizana nawo.Indirect immunoperoxidase staining and flow cytometry yokhala ndi mAb 8281 inawonetsa madontho abwino pamatenda ambiri, kuphatikiza minofu yosalala, m'matumbo am'mimba, ma lymph node B cell ndi ma monocyte.Kusanthula kwa ITLC pakupanga kwa GSL kwa ma neoplasms atsopano a B cell pogwiritsa ntchito mAb 8281 kunatsimikizira kukhalapo kwa lactosylceramide ndi galactosylceramide mu ma neoplasms a magawo osiyanasiyana osiyanitsa.Chifukwa cha kutsimikizika kwake kwa zotsalira za βGal carbohydrate, mAb 8281 ikhoza kukhala yothandiza pakuwunika kwamachitidwe ndi magwiridwe antchito a GSL.
