Zogulitsa

Zochitika zamoyo: BCIP (BCIP p-toluidine mchere; X-phosphate p-toluidine mchere) ndi gawo lapansi la chromogenic pozindikira zochita za alkaline phosphatase.

Shiga poizoni (Stxs) amapangidwa ndi enterohemorrhagic Escherichia coli (EHEC), yomwe imayambitsa matenda omwe nthawi zambiri amapha anthu.Mizere ya cell ya Vero, yochokera ku impso zobiriwira za nyani za ku Africa, zimayimira mulingo wagolide wodziwira zotsatira za cytotoxic za Stxs.Ngakhale kuti akugwiritsidwa ntchito padziko lonse lapansi, chidziwitso chokhudza momwe ma Stx receptor glycosphingolipids (GSLs) ndi msonkhano wawo muzitsulo zamadzimadzi ndizochepa.Apa tikuwonetsa kusanthula kwatsatanetsatane kwa ma Stx receptor GSL ndi kugawa kwawo ku nembanemba zosamva zotsekemera (DRMs), zomwe zidakonzedwa kuchokera ku maselo a Vero-B4 ndikugwiritsiridwa ntchito ngati lipid raft raft.Tinazindikira globotriaosylceramide (Gb3Cer) ndi globotetraosylceramide (Gb4Cer) ngati zolandilira za GSL za Stx1a, Stx2a, ndi Stx2e subtypes pogwiritsa ntchito kuzindikira kwa pamwamba pa TLC kuphatikiza ndi MS.Stx receptor yachilendo, globopentaosylceramide (Gb5Cer, Galβ3GalNAcβ3Galα4Galβ4Glcβ1Cer), yomwe inadziwika bwino (kuphatikizapo Gb3Cer ndi Gb4Cer) ndi Stx2e, inali yodziwika bwino.Ma Lipoforms a Stx receptor GSLs adapezeka kuti amakhala makamaka ndi ceramide moieties wopangidwa ndi sphingosine (d18: 1) ndi C24: 0 / C24: 1 kapena C16: 0 mafuta acid.Kuphatikiza apo, zomwe zimachitika limodzi ndi zolembera za lipid raft, SM ndi cholesterol, mu DRMs zikuwonetsa kuyanjana kwa GSL ndi ma membrane microdomain.Phunziroli limapereka maziko owunikiranso momwe magwiridwe antchito a lipid raft-associated Stx receptors chifukwa cha kuvulala koopsa kwa ma cell a Vero-B4.