Ammonium hexachloroiridate(IV) CAS:16940-92-4
| Nambala ya Catalog | XD90606 |
| Dzina lazogulitsa | Ammonium hexachloroiridate (IV) |
| CAS | 16940-92-4 |
| Molecular Formula | H8Cl6IrN2 |
| Kulemera kwa Maselo | 441.012 |
| Zambiri Zosungira | Wozungulira |
Mafotokozedwe a Zamalonda
| Maonekedwe | Makhiristo obiriwira a azitona |
| Kuyesa | 99% |
Kuwonongeka kwa okosijeni ku maziko a DNA nthawi zambiri kumapangitsa kupanga ma purines okosijeni, makamaka 7,8-dihydro-8-oxoguanine (8-oxoG) ndi 7,8-dihydro-8-oxoadenine (8-oxoA), yomwe kale inali yabwino. chodziwika mutagenic chotupa.Popeza 8-oxoG imakhudzidwa mosavuta ndi okosijeni wowonjezereka poyerekeza ndi maziko abwino, kuyika maziko panthawi ya DNA kaphatikizidwe motsutsana ndi mawonekedwe a oxidized a 8-oxoG adafufuzidwa mu vitro.Template yopangira yokhala ndi chotupa chimodzi cha 8-oxoG idayamba kuthandizidwa ndi ma elekitironi amtundu umodzi kapena pansi pamikhalidwe ya okosijeni imodzi ndipo kenako idalumikizidwa ndi gawo la Klenow fragment exo- (Kf exo-), ng'ombe thymus DNA polymerase alpha (pol alpha). ) kapena DNA polymerase beta (pol beta).Mogwirizana ndi malipoti am'mbuyomu, dAMP ndi dCMP zimayikidwa mosankha moyang'anizana ndi 8-oxoG ndi ma DNA polymerases onse atatu.Chochititsa chidwi, oxidation ya 8-oxoG inapezedwa kuti ipangitse kuyika kwa dAMP ndi dGMP moyang'anizana ndi chotupa ndi Kf exo- ndi kuletsa kwakanthawi kwa primer extension o curring pa malo osinthidwa maziko.Kuphatikiza apo, chotupacho chimapanga chipika pakaphatikizidwe ka DNA ndi pol alpha ndi pol beta.Kuyesera ndi 8-oxoA-modified template oligonucleotide amasonyeza kuti 8-oxoA ndi mawonekedwe oxidized a 8-oxoA kuika mwachindunji dTMP ndi Kf exo-.Kusanthula kwakukulu kwa 8-oxoG-containing oligonucleotides isanayambe ndi pambuyo pa okosijeni ndi IrCl62-amagwirizana ndi okosijeni makamaka pa malo a 8-oxoG, zomwe zimapangitsa kuti pakhale guanidinohydantoin moiety monga chinthu chachikulu.Palibe umboni wopangira masamba oyambira omwe adapezeka.Zotsatira izi zikuwonetsa kuti mawonekedwe a okosijeni a 8-oxoG, mwina guanidinohydantoin, angatsogolere kuwerengera molakwika ndi kusokoneza ma dNTP panthawi ya kaphatikizidwe ka DNA.Ngati njira yotereyi inachitika mu vivo, imayimira zilonda za mutagenic zomwe zimatsogolera ku kusintha kwa G-->T ndi G-->C.Komabe, mawonekedwe ofanana ndi okosijeni a 8-oxoA makamaka amawonetsa kuyika kolondola kwa T panthawi ya DNA kaphatikizidwe ndi Kf exo-.
