Zogulitsa

Kachilombo ka electrochemiluminescence (ECL) wopanda label-free electrochemiluminescence (ECL) immunosensor wa ultrasensitive komanso kuzindikira mwachangu mabakiteriya a m'madzi a Vibrio parahaemolyticus (VP) m'madzi am'nyanja ndi nsomba zam'nyanja adapangidwa kutengera ma graphene oxide amitundu yambiri, omwe adakonzedwa ndi N-(4-aminobutyl) -N -ethylisoluminol (ABEI) ndi VP antibody (anti-VP) panthawi imodzimodziyo osasunthika pamtunda wa maginito a graphene oxide (nanoFe3O4@GO).ABEI ndi anti-VP adakhala ngati electrochemiluminophore ndi chipangizo chojambula cha VP motsatira.Mayendedwe abwino komanso mawonekedwe a mbali ziwiri a nanoFe3O4@GO adathandizira ABEI yonse, yosasunthika pa GO, yogwira ntchito ndi electrochemically ndipo motero kumathandizira kuzindikira.Pazifukwa zabwino, mphamvu ya ECL inatsika ndi kuwonjezeka kwa logarithmic kwa VP mumtundu wa 10-10 (8) CFU/mL, ndi malire a 5CFU / mL a madzi a m'nyanja ndi 5CFU / g pazakudya zam'nyanja.ECL immunosensor iyi inawonetsa kutsimikizika kwakukulu, kukhazikika komanso kuberekana kuti azindikire VP.Kuonjezera apo, ECL immunosensor yakhala ikugwiritsidwa ntchito bwino kuti mudziwe kuchuluka kwa VP m'madzi a m'nyanja ndi m'nyanja mofulumira, ndikuchira kwa 94.4-112.0% ndi RSD 4.1-11.7%.Chifukwa chake, ma immunosensor opangidwa amawonetsa chiyembekezo chachikulu chogwiritsa ntchito.