Zogulitsa

Kuzindikira kwamakina mu banja lodalira la Ca2+ la alpha-mannosidases mu symbiont yamatumbo amunthu.

Mabakiteriya a Colonic, owonetseredwa ndi Bacteroides thetaiotaomicron, amagwira ntchito yofunika kwambiri posunga thanzi laumunthu pogwiritsa ntchito mabanja akuluakulu a glycoside hydrolases (GHs) kuti agwiritse ntchito ma polysaccharides a zakudya ndi ma glycans monga zakudya.Kukula kwa banja la GH koteroko kumasonyezedwa ndi 23 banja la GH92 glycosidases lolembedwa ndi B. thetaiotaomicron genome.Apa tikuwonetsa kuti awa ndi ma alpha-mannosidase omwe amagwira ntchito pogwiritsa ntchito njira imodzi yosamuka kuti agwiritse ntchito N-glycans.Mapangidwe atatu amtundu wa mannosidase awiri a GH92 amatanthawuza banja la mapuloteni awiri omwe ali ndi malo opangira malo, kupereka chithandizo cha asidi (glutamate) ndi maziko (aspartate) ku hydrolysis mu Ca (2+) - njira yodalira.Mapangidwe amitundu itatu a GH92s ovuta okhala ndi zoletsa amapereka chidziwitso chatsatanetsatane, makina ndi njira yofananira ya catalysis.Ca(2+) imagwira ntchito yofunika kwambiri pothandizira kupotoza mannoside kutali ndi mawonekedwe ake apansi (4)C(1) pampando kudera la kusintha.（Bibliography: Nat.Chemu.Bioli.6, 125-32, (2010)

Frontal affinity chromatography ya ovalbumin glycoasparagines pagawo la concanavalin A-sepharose.Kafukufuku wochulukira wa zomwe zimamangiriza za lectin.

Kuyanjana kwa Sepharose 4B-immobilized concanavalin A (ConA) ndi 10 glycoasparagines yochokera ku ovalbumin adafufuzidwa mochulukira ndi frontal affinity chromatography.Mwanjira iyi, yankho la carbohydrate limagwiritsidwa ntchito mosalekeza ku gawo la ConA-Sepharose ndipo kubwezeredwa kwa kutsogolo kumayesedwa ngati gawo la mphamvu ya kuyanjana.The dissociation constant (Kd) ya saccharide iliyonse yokhala ndi ConA imatha kuzindikirika.Kuwunika kwa kumangiriza kwa p-nitrophenyl-alpha,D-mannoside kwawonetsa kuti zomwe zimamangiriza za ConA sizisintha kwenikweni pambuyo pokhazikika pa Sepharose 4B.Iliyonse ya ovalbumin glycoasparagines idalembedwa ndi tritium ndi njira yochepetsera ya methylation yowunikira.Kuyerekeza kwa ma Kd omwe adapezedwa adawonetsa kuti kumangirira kwa ConA kumasiyana kwambiri ndi kusiyana pang'ono kwamapangidwe a unyolo wa glycosyl.Zotsatira zikuwonetsa kuti ConA imazindikira kapangidwe kake kake ka glycosyl, Man alpha 1-6 (Man alpha 1-3)Man, momwe gulu limodzi la hydroxyl pa C-3 malo a C-6-linked mannose liyenera kukhala laulere.