Zogulitsa

Chemifluorescence: Molecular luminol ndi molekyulu ya fluorescent yomwe imatha kusinthidwa kukhala okondwa aminophthalic acid pamaso pa mamolekyu a hydrogen peroxide, omwe amatulutsa fluorescence yamphamvu.Hydrogen peroxide idapangidwa ndi machitidwe ambiri a biooxidative, kotero ndikosavuta kulumikiza machitidwe a biooxidative ndi photodetection poyambitsa luminol.Mwachitsanzo, kafukufuku wa glucose oxidase/catalase amatha kudziwa kuchuluka kwa hydrogen peroxide kapena shuga mu zitsanzo, ndipo nthawi yoyankha ndi ma 0.5s okha (njira yamphamvu).Kuphatikiza zinthu za chemiluminescent ndi machitidwe a immunological, ndikuwonetsa kuchuluka kwa zigawo zomwe zapezeka ndi chitetezo chamthupi pochita kuwala, zimatchedwa ukadaulo wa chemiluminescent immunoassay.Mu 1976, Shmeder adapempha chemiluminescence immunoassay, pogwiritsa ntchito luminol-H202 ndi zochokera ku ABEI monga njira yowonetsera.Pakalipano, luminol ndi zotuluka zake ndizo zolembera zomwe zimagwiritsidwa ntchito kwambiri mu chemiluminescence immunoassay applications.Pansi pamikhalidwe yamchere, yopangidwa ndi microperoxidase, ma photon ambiri amatha kutulutsidwa.Luminol itha kugwiritsidwa ntchito pa WesternBlot ya ma antibodies olembedwa ndi HRP ndi nucleic acid hybridization kuzindikira kwa ma probe olembedwa ndi HRP.Amagwiritsidwanso ntchito pozindikira matenda amtundu wamagazi amakono.Chigawo chomwe chingagwiritsidwe ntchito ngati chemiluminescent marker chiyenera kukwaniritsa zotsatirazi: quantum zokolola za luminescence ndizokwera;katundu wake physicochemical amafanana dongosolo pansi kuphunzira;mawonekedwe ake kuwala ndi zotsatira za makutidwe ndi okosijeni wa zinthu luminescent;mkati mwa ndende zomwe zimagwiritsidwa ntchito Mkati zopanda poizoni kwa zamoyo.Mitundu yambiri ya chemiluminescence reagents imagwiritsidwa ntchito kwambiri: acridine ester, chemiluminescence tracer yomwe imagwiritsidwa ntchito kwambiri, ndi tricyclic organic pawiri, yosavuta oxidize, ndipo makutidwe ndi okosijeni safuna chothandizira, ndipo amatulutsa photon pa 430nm;Luminol ndi isoluminol ndi zotumphukira zawo ndi okhwima kwambiri chemiluminescent agents.Kale mu 1964, Ngakhale lipoti la chemiluminescence, luminol, isoluminol ndi zotumphukira zake zidagwiritsidwa ntchito pa chemiluminescence.Ma immunoassays apeza zotsatira zabwino.Kuzindikira kwa luminescence: mawonekedwe abwino kwambiri a fluorescence wavelength ndi 400nm (kuzindikira chemiluminescence mu 60mmK2S2O8, 100mmK2CO3, pH11.5 yankho)

Chemiluminescence: Monga njira yowunikira, chemiluminescence imagwiritsidwa ntchito kwambiri pozindikira ma free radicals ndi reaction metabolites opangidwa mu michere, ma cell ndi zamoyo.Kuwala komwe kumapangidwa ndi ma oxidation awo ndi metabolites kumatha kugwiritsidwa ntchito ndi ma photometer osiyanasiyana.kuzindikira.Chemiluminescence imagwiritsidwa ntchito kwambiri pofufuza ndi kufufuza za antioxidants, monga polyphenols, polysaccharides, flavonoids, ndi anthraquinones, chifukwa cha kukhudzidwa kwake, kufulumira, ntchito yosavuta komanso mtengo wotsika.Makina omwe amagwiritsidwa ntchito kwambiri pa chemiluminescence pozindikira ma anions a superoxide ndi xanthine oxidase-luminol, pyrogallol-luminol ndi dimethyl sulfoxide-luminol.Yoyamba ndi dongosolo la enzymatic, ndipo awiri omalizira Ndi dongosolo lopanda enzymatic.Makina a chemiluminescence ozindikira ma hydroxyl radicals makamaka amaphatikiza yisiti ya copper sulfate (kapena maselo am'mafupa) -ascorbic acid-hydrogen peroxide, CuCl-H2O2-o-phenanthroline-carbonate buffer, copper sulfate-o-phenanthroline-ascorbic acid-hydrogen. peroxide 5 chemiluminescence machitidwe, ferrous sulfate-luminol-hydrogen peroxide ndi ferrous sulfate-luminol, onse amakhudza tingachipeze powerenga Fenton anachita kupanga hydroxyl ankafuna kusintha zinthu mopitirira, ndiyeno kuukira wothandizila luminescent kupanga chemiluminescence, amene angagwiritsidwe ntchito kuyeza kuchotsa akupanga. ntchito ya reactive oxygen radicals.Njira yoyesera: gwiritsani ntchito pyrogallol-luminol chemiluminescence system: luminol imapangidwa kukhala 0.05mol/L solution ndi 0.05mol/L NaOH solution, yosungidwa pamalo amdima, ndi madzi osungunuka kawiri musanagwiritse ntchito Dilute mpaka 1mmol/L solution, gwiritsani ntchito 1mmol/ L HCl kuti mupange 0.01mol / L yankho la pyrogallol, sungani mufiriji pa 4 ° C, ndikuwatsitsa ndi madzi osungunuka kawiri mpaka 16 nthawi 6.25 × 10-4mol / L yankho musanagwiritse ntchito.0.05mol / L pH10.2Na2CO3-NaHCO3 chotchinga (chokhala ndi 0.1mmol / LEDTA) chinakonzedwa musanagwiritse ntchito, ndikusakaniza ndi 1mmol / L luminol pa 2: 1 (gawo la voliyumu) ​​kuti apange luminol ndi carbonate buffer pamaso pa osakaniza kuyesera.Pa kuyeza, jekeseni 10.0 μL zitsanzo za ndende zosiyanasiyana (0, 0.08, 0.4, 2 ndi 10 mg/mL) mu selo luminescent (ndi buffer chitsanzo monga ulamuliro), ndiyeno jekeseni 6.25 × 10-4mol/L pyrogallol. 0.05 Pomaliza, 0.94 mL ya chisakanizo cha luminol ndi carbonic acid buffer adawonjezeredwa kuti ayambitse (30 ° C), mphamvu ya luminescence idawerengedwa pakapita nthawi 2 s, ndipo mphamvu yonse yophatikizika ya luminescence pa 300 s idayesedwa.Kuchuluka kwa luminescence yakumbuyo kunali luminescence pomwe pyrogallol sinawonjezedwe.mtengo.Kuonjezera apo, pali chemiluminescence, fluorescence spectroscopy ndi NBS-dichlorofluorescein chemiluminescence system pofuna kudziwa fluorescence chemiluminescence, etc., ubwino wawo waukulu ndi kutengeka kwakukulu.

Chemical Properties: Yellow crystalline ufa.Amasungunuka mosavuta mu lye, amasungunuka mu asidi osungunuka, pafupifupi osasungunuka m'madzi, komanso osasungunuka mu mowa.Mayankho osalowerera ndale kapena acidic pang'ono amawonetsa kuwala kowala kwabuluu akamawunikira kuwala kwa ultraviolet.Malo osungunuka 329-332 ℃

Ntchito: Monga mankhwala kusanthula reagent ndi chizindikiro.Gwiritsani ntchito Chemiluminescence analysis reagent (monga kutsimikiza kwa zitsulo kapena magazi)

Ntchito: Kusanthula chemiluminescence, monga: zitsulo cations, magazi ndi glucocorticoids.

Ntchito: Luminescence test: Emmax440nm (chemiluminescence; 60mmK2S2O8, 100mmK2CO3, pH11.5; pambuyo kuwonjezera H2O2) chemiluminescence reagent ndi chizindikiro, ambiri ntchito kusanthula chemiluminescence, monga cations zitsulo, magazi chitetezo chokwanira, etc.