Zogulitsa

2,3,4,6-Tetrakis-O-trimethylsilyl-D-gluconolactone, yomwe imadziwika kuti TMS-D-glucose, ndi yosunthika yomwe imapeza ntchito m'madera osiyanasiyana a sayansi, kuphatikizapo organic synthesis, carbohydrate chemistry, ndi analytical chemistry. TMS-D-glucose ndi yofunika kwambiri mu kaphatikizidwe ka organic chifukwa imagwira ntchito ngati gulu loteteza magulu a hydroxyl (OH) omwe amagwira ntchito muzakudya.Poyambitsa magulu a trimethylsilyl (TMS) pamagulu a hydroxyl a shuga, chigawocho chimakhala chokhazikika komanso chosasunthika, zomwe zimapangitsa kuti magulu enaake a hydroxyl asinthe ndikusiya ena osakhudzidwa panthawi ya kusintha kwa mankhwala.Njira yodzitchinjiriza iyi imagwiritsidwa ntchito kwambiri muzakudya zama carbohydrate kuti akwaniritse regioselectivity ndi stereochemistry pakuphatikizika kwamafuta ovuta, glycoconjugates, ndi zinthu zachilengedwe. za carbohydrate.Mwa kutembenuza ma carbohydrate kukhala zotumphukira zawo za trimethylsilyl, kusasunthika kwawo ndi kukhazikika kwa kutentha kumapita patsogolo, kuwapangitsa kukhala oyenera kuwunika ndi gas chromatography (GC) ndi mass spectrometry (MS).Njira yotulutsirayi imakulitsa chidwi chodziwikiratu, imapangitsa kuti pakhale mphamvu zolekanitsa, komanso imathandizira kuzindikira ma carbohydrate osiyanasiyana muzosakaniza zovuta, monga zitsanzo zamoyo kapena zinthu zazakudya.TMS-D-glucose imapezanso ntchito pakuphatikizika kwa ma reagents apadera ndi kafukufuku wamankhwala.Kukhazikika kwake kwapadera komanso kukhazikika kumapangitsa kukhala chinthu chofunikira poyambira pokonzekera mankhwala ena opangidwa ndi ma carbohydrate.Ochita kafukufuku amatha kusintha trimethylsilyl moiety kapena kulowetsamo glucose moiety kuti apange mankhwala okhala ndi zinthu zinazake, monga ma probes a fulorosenti, ma enzyme inhibitors, kapena ofuna mankhwala.Zotengera izi zitha kugwiritsidwa ntchito m'maphunziro osiyanasiyana achilengedwe komanso zamankhwala, kuphatikiza kujambula, kukula kwa mankhwala, kapena kumvetsetsa kuyanjana kwa mapuloteni a carbohydrate. kusamalitsa.Ochita kafukufuku ayenera kuonetsetsa kuti mpweya wabwino umakhala wokwanira komanso kugwiritsa ntchito zida zoyenera zodzitetezera pogwira ntchito ndi mankhwalawa kuti apewe ngozi zomwe zingachitike paumoyo.Kuonjezera apo, monga momwe zimakhalira ndi mankhwala enaake, kuyera, ndi khalidwe la TMS-D-shuga ndizofunikira kwambiri kuti mupeze zotsatira zodalirika komanso zowonjezera.Kutha kwake kuteteza magulu a hydroxyl muzakudya, kugwiritsa ntchito kwake pakuwunika kwamafuta, komanso kugwiritsidwa ntchito kwake pakuphatikiza ma reagents apadera kumapangitsa kuti ikhale chida chofunikira pamagawo osiyanasiyana asayansi.Pogwiritsa ntchito TMS-D-glucose, ofufuza atha kupititsa patsogolo maphunziro awo muzakudya zama carbohydrate, glycoscience, ndi magawo ena okhudzana ndi izi, zomwe zimathandizira kupanga zinthu zatsopano, zowunikira, ndi zochizira.