Mapuloteni opanga ma sign a PII amafalikira kwambiri mu prokaryotes ndi zomera komwe amawongolera kuchuluka kwa machitidwe a anabolic.Kuchita bwino kwa metabolites kumafuna kupumula mabwalo owongolera ma cell.Apa tikuwonetsa kuti kusintha kwa mfundo imodzi mu mapuloteni owonetsa PII kuchokera ku cyanobacterium Synechocystis sp.PCC 6803 ndi yokwanira kuti mutsegule njira ya arginine yomwe imachititsa kuti biopolymer cyanophycin (multi-L-arginyl-poly-L-aspartate) ikhale yokwanira.Chogulitsachi ndi cha chidwi cha biotechnological monga gwero la amino acid ndi polyaspartic acid.Ntchitoyi ikupereka chitsanzo cha njira yatsopano yopangira uinjiniya popanga mapuloteni ozindikiritsa a PII opangidwa ndi makonda.Apa, opangidwa ndi Synechocystis sp.PCC6803 strain yokhala ndi PII-I86N mutation over-accumulated arginine kudzera mu activation ya key enzyme N-acetylglutamate kinase (NAGK) . kuposa mu mtundu wakuthengo.Zotsatira zake, mtundu wa BW86 udawunjikana mpaka 57 % ya cyanophycin pa cell youma mumiyezo yoyesedwa, yomwe ndi yokolola kwambiri ya cyanophycin yomwe idanenedwapo pano.Kupsyinjika kwa BW86 kunapanga cyanophycin mu molekyulu yochuluka ya 25 mpaka> 100 kDa;mtundu wamtchire umatulutsa polima mumitundu yosiyanasiyana ya 30 mpaka > 100 kDa. Zokolola zambiri komanso kuchuluka kwa ma molekyulu a cyanophycin opangidwa ndi strain BW86 pamodzi ndi zofunikira zochepa za michere ya cyanobacteria zimapangitsa kuti ikhale njira yodalirika yopangira biotechnological kupanga cyanophycin.Kafukufukuyu akuwonetsanso kuthekera kwa metabolic pathway engineering pogwiritsa ntchito mapuloteni ozindikiritsa a PII, omwe amapezeka m'mabakiteriya ambiri.
